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US 9,284,547 B2Granted
Methods for detecting modification resistant nucleic acids
AssigneeThe Regents of the University of California
InventorsJonathan Weissman, Nicholas Ingolia, Sina Ghaemmaghami, John Newman
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Abstract
Methods are provided for, inter alia, detecting nucleic acid molecules resistant to degradation, such as a plurality of RNA molecules bound to a ribosome, using various technologies including deep sequencing.
Claims20
A method for determining a protein synthesis rate, comprising:
- (a) isolating a plurality of monosomes from a plurality of polysomes, wherein each of said plurality of polysomes comprises a ribosome bound to a portion of a translatable RNA molecule;
- (b) sequencing each of said portions;
- (c) determining a ribosome footprint density for each translatable RNA molecule from said sequencing;
- and
- (d) determining a protein synthesis rate from the ribosomal footprint density.
2 more elements
A method for determining the protein synthesis rate for a plurality of protein coding genes, comprising:
- (a) isolating a plurality of monosomes from a plurality of polysomes, wherein each of said plurality of polysomes comprises a plurality of ribosomes, wherein each of said plurality of ribosomes is bound to a portion of a mRNA molecule;
- (b) aligning the sequence of each of said portions;
- and
- (c) determining the ribosomal footprint density from said aligning, thereby determining the protein synthesis rate for a plurality of protein coding genes.
1 more element
Dependencies are inferred from the claim text.