Neutral active soluble truncated PH20 polypeptides and uses thereof
AssigneeHalozyme, Inc.
InventorsGe Wei, Krishnasamy Panneer Selvam, Louis Bookbinder, Gregory I. Frost
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Abstract
Soluble PH20 polypeptides are provided, including extended soluble PH20 polypeptides, and uses thereof. Also provided are other C-terminally truncated PH20 polypeptides and partially deglycosylated PH20 polypeptides and uses thereof.
Claims22
A truncated soluble PH20 hyaluronidase, wherein:
- the truncated PH20 hyaluronidase is neutral active;
- is soluble;
- and
- consists of any sequence of amino acid residues within SEQ NO:107 that terminates at residue 469, 470 or 471 of SEQ ID NO:107.
A truncated soluble PH20 hyaluronidase that is selected from among a polypeptide that contains an amino acid substitution or amino acid substitutions in the sequence of amino acids set forth as amino acids 36-469, 36-470 or 36-471 of SEQ ID NO:
- 107 that has at least 95% sequence identity with the sequence of amino acids set forth as amino acids 36-469, 36-470 or 36-471 of SEQ ID NO:
- 107, wherein:
- the amino acid sequence of the amino acid-substituted polypeptide differs from the sequence of amino acids set forth as amino acids 36-469, 36-470 or 36-471 of SEQ ID NO:
- 107 only by amino acid substitutions;
- the polypeptide is soluble;
- and
- the polypeptide has hyaluronidase activity.
A truncated soluble PH20 hyaluronidase that consists of the sequence of amino acids set forth as amino acids 36-469, 36-470 or 36-471 of SEQ ID NO:
- 107.
Dependencies are inferred from the claim text.