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US 9,284,527 B2Granted
Use of stem cell conditioned medium to inhibit melanin formation for skin whitening
AssigneeGrowgene Biotech Inc.
InventorsPei-Chuan Chuang, Huei-Chun Liu
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Abstract
A use of a stem cell conditioned medium to inhibit melanin formation for skin whitening is revealed. First, mesenchymal stem cells are cultured in a cell culture dish containing complete growth media. After mesenchymal stem cells are sub-cultured in a complete growth media for three times, a conditioned medium can be acquired from the basal media.
Claims3
A method of whitening skin using a stem cell conditioned medium, comprising:
- applying at least 2.5 wt. % of the stem cell conditioned medium to the skin of a subject in need thereof so as to decrease tyrosinase activity and melanin synthesis thereby whitening the skin;
- and wherein said stem cell conditioned medium is produced by (a) culturing human Wharton's jelly mesenchymal stem cells in a cell culture dish containing a complete growth medium, wherein the complete growth medium includes α-MEM, fetal bovine serum, and human-basic fibroblast growth factors;
- and (b) sub-culturing the Wharton's jelly mesenchymal stem cells in the complete growth medium and transferring the stem cells to a basal medium to obtain said stem cell conditioned medium, wherein the basal medium includes α-MEM and human-basic fibroblast growth factors.
Dependencies are inferred from the claim text.