Premounted fluid conveyance assembly for cell expansion system and method of use associated therewith

A premounted fluid conveyance assembly for use with a cell expansion machine, the cell expansion machine including at least one valve clamp and a rotatable shaft, the premounted fluid conveyance assembly comprising:

• a fluid conveyance system comprising:
• a gas transfer module;
• a length of tubing fluidly associated with the gas transfer module;
• a rotatable hollow fiber bioreactor fluidly associated with the length of tubing and the gas transfer module;
• a bioreactor coupling comprising a shaft fitting, wherein the bioreactor coupling is coupled to the rotatable hollow fiber bioreactor and the shaft fitting comprises at least a spring member for engaging with the rotatable shaft of the cell expansion machine to rotate the rotatable hollow fiber bioreactor;
• a support member including a plurality of holding elements, the plurality of holding elements including a gas transfer module mounting post and a plurality of tubing guides holding at least a portion of the length of tubing, wherein the support member further comprises a shaft access aperture that is oblong shaped to mate with a fitting on the rotatable shaft of the cell expansion machine and the support member further comprising a plurality of sensor ports to provide access between pressure and temperature sensors and sensor couplings;
• a plurality of pump tubing conduits, at least a portion of each of the plurality of pump tubing conduits extending beyond the support member and looping back to a perimeter of the support member, wherein each of the plurality of pump tubing conduits is engageable with at least one of an IC circulation pump, an EC circulation pump, an IC inlet pump, or an IC inlet pump on a cell expansion machine;
• and
• wherein the support member and the fluid conveyance system are adapted to be detachably-attached to the cell expansion machine.

A premounted fluid conveyance assembly for use with a cell expansion machine, the cell expansion machine including at least one valve clamp, at least one pump, and a rotatable shaft associated within a shaped fitting, the premounted assembly comprising:

• a fluid conveyance system comprising:
• a gas transfer module;
• a length of tubing fluidly associated with the gas transfer module;
• a rotatable hollow fiber bioreactor fluidly associated with the length of tubing and the gas transfer module;
• a bioreactor coupling comprising a shaft fitting, wherein the bioreactor coupling is coupled to the rotatable hollow fiber bioreactor and the shaft fitting comprises a spring member for engaging with the rotatable shaft of the cell expansion machine to rotate the rotatable hollow fiber bioreactor;
• a means for supporting at least a portion of the fluid conveyance system, wherein the means for supporting comprises:
• a shaped access aperture that is oblong shaped to mate with a fitting on the rotatable shaft of the cell expansion machine;
• a plurality of sensor ports to provide access between pressure and temperature sensors and sensor couplings;
• a means for detachably-attaching the means for supporting to the cell expansion machine;
• a means for holding the gas transfer module;
• and
• a means for holding the length of tubing fluidly associated with the gas transfer module;
• and
• a plurality of pump tubing conduits, at least a portion of each of the plurality of pump tubing conduits extending beyond the means for supporting and looping back to a perimeter of the means for supporting, wherein each of the plurality of pump tubing conduits is engageable with at least one of an IC circulation pump, an EC circulation pump, an IC inlet pump, or an IC inlet pump on the cell expansion machine.