Method of producing heterogeneous protein
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsHisahiro Tabuchi, Tomoya Sugiyama
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Abstract
The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.
Claims13
A method of producing an antibody, comprising culturing an animal cell which expresses alanine aminotransferase and has a transferred DNA encoding the antibody and thereby allowing the animal cell to produce the antibody,
- wherein the animal cell is a cell transformed with DNA encoding the alanine aminotransferase;
- and
- wherein the alanine aminotransferase is any one of the following (a)-(c):
- (a) a polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 10;
- (b) a polypeptide which has an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO:
- 10 by substitution, deletion, addition and/or insertion of 1-5 amino acid residues and yet has alanine aminotransferase activity;
- and
- (c) a polypeptide having 99% or more amino acid sequence homology with the amino acid sequence as shown in SEQ ID NO:
- 10 and yet having alanine aminotransferase activity.
A cultured cell transformed with DNA encoding a polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 10 and a transferred DNA encoding a desired antibody.
A cultured cell transformed with DNA encoding a polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 10 and DNA encoding a taurine transporter.
A method of producing a desired polypeptide, comprising culturing in an α-ketoglutarate-containing medium a cell transformed with DNA encoding alanine aminotransferase and which has a transferred DNA encoding the desired polypeptide and thereby allowing the cell to produce said desired polypeptide, wherein the alanine aminotransferase is any one of the following (a)-(c):
- (a) a polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 10;
- (b) a polypeptide which has an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO:
- 10 by substitution, deletion, addition and/or insertion of 1-5 amino acid residues and yet has alanine aminotransferase activity;
- and
- (c) a polypeptide having 99% or more amino acid sequence homology with the amino acid sequence as shown in SEQ ID NO:
- 10.
A method of producing an antibody, comprising culturing an animal cell which expresses alanine aminotransferase and has a transferred DNA encoding the antibody and thereby allowing the animal cell to produce the antibody,
- wherein the animal cell is a cell transformed with exogenous DNA encoding the alanine aminotransferase;
- and
- wherein the alanine aminotransferase is a hamster aminotransferase.
A cultured cell transformed with exogenous DNA encoding a hamster alanine aminotransferase and a transferred DNA encoding a desired antibody.
A cultured cell transformed with exogenous DNA encoding a hamster alanine aminotransferase and DNA encoding a taurine transporter.
A method of producing a desired polypeptide, comprising culturing in an α-ketoglutarate-containing medium a cell transformed with exogenous DNA encoding alanine aminotransferase and which has a transferred DNA encoding the desired polypeptide and thereby allowing the cell to produce said desired polypeptide, wherein the alanine aminotransferase is a hamster alanine aminotransferase.
Dependencies are inferred from the claim text.