Methods of producing adalimumab
AssigneeAbbVie Inc.
InventorsItzcoatl A. Pla, Joseph G. Matuck, John C. Fann, Christof Schulz, Nichole A. Roy, David F. Bruton, James McIntire, Yu-Hsiang D. Chang, Thomas Seewoester
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Abstract
The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
Claims30
A method of producing adalimumab, comprising culturing in large-scale mammalian cells that express adalimumab in a cell culture production medium, wherein the pH of the cell culture production medium is adjusted from a first pH to a second pH during said culturing, the second pH being lower than the first pH, and the cell culture production medium is not removed but is supplemented with glucose or one or more other nutrients at least once during adalimumab production;
- and wherein adalimumab produced by the mammalian cells is purified from the cell culture production medium using a process including Protein A affinity purification.
A method of producing adalimumab, comprising culturing in large-scale mammalian cells that express adalimumab in a cell culture production medium, wherein during adalimumab production, the mammalian cells are initially cultured at a first temperature and then cultured at a reduced temperature, and the cell culture production medium is not removed but is supplemented with a nutrient at least once;
- and the adalimumab produced by the mammalian cells is purified from the cell culture production medium using a process including Protein A affinity purification.
A method of producing adalimumab, comprising culturing in large-scale mammalian cells that express adalimumab in a cell culture production medium, wherein a) the mammalian cells are initially cultured at a first pH and then cultured at a reduced pH, wherein the first pH is 8 or less and the reduced pH is 6.5 to 7;
- b) during adalimumab production the mammalian cells are initially cultured at a first temperature and then cultured at a reduced temperature;
- c) during adalimumab production, the culture medium is not removed but is supplemented with glucose;
- d) during adalimumab production, the culture medium is supplemented with a nutrient other than glucose at least once;
- and e) the adalimumab produced by the mammalian cells is purified from the cell culture production medium using a process including Protein A affinity purification.
Dependencies are inferred from the claim text.