Preparative chromatography column and methods

A disposable preparative chromatography column for effecting separation of components of a process liquid comprising:

• a. a flexible disposable bag with an inner volume;
• b. a septum disposed within the flexible disposable bag to separate the flexible disposable bag into a flexible upper chamber and a flexible lower chamber, the septum including a porous surface that allows passage of liquid but not chromatography media from the upper chamber to the lower chamber;
• c. a chromatography media disposed in the upper chamber;
• d. at least one inlet to introduce liquid into the flexible upper chamber of the bag containing the chromatography media;
• e. at least one outlet to remove the liquid from the flexible lower chamber of the bag after passage through the septum;
• and
• f. at least one mixing mechanism to mix the contents of the bag.