Passive microfluidic metering device

An integrated fluidic device comprising:

• a sample fluid input chamber configured to provide an input of a sample fluid;
• a first overspill chamber in fluid communication with the fluid input chamber;
• a metering conduit in fluid communication with the fluid input chamber and the first overspill chamber, wherein the metering conduit is configured to meter a first metered volume of fluid from the sample fluid, and the first overspill chamber is configured to receive fluid from the sample fluid in excess of the first metered volume of fluid;
• and
• a second overspill chamber in fluid communication with the metering conduit, wherein the metering conduit is configured to meter a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber is configured to receive fluid from the first metered volume of fluid in excess of the second metered volume of fluid;
• wherein the second overspill chamber has a fluid actuated closable valve configured to isolate the second metered volume of fluid out of the first metered volume of fluid when the fluid actuated closable valve closes;
• and
• wherein the first overspill chamber includes a first absorbent pad configured to absorb the fluid in excess of the first metered volume of fluid, and the fluid actuated closeable valve includes a second absorbent pad configured to absorb the fluid in excess of the second metered volume of fluid.