Lid for functionalized microfluidic platform and method

A lid, for a functionalized microfluidic platform, the microfluidic platform including a base having outer surface and a channel therethough for receiving fluid therein, the channel having input and output ports communicating with the outer surface, comprising:

• a body having a first well extending therethrough, the first well including first and second ports communicating therewith and being adapted for receiving a first substance therein;
• a first membrane extending over the first port of the lid, the first membrane retaining the first substance in the first well and allowing a second substance to diffuse therethrough into the first well;
• a removable seal releaseably connected to at least one of the first membrane and the body adjacent the first membrane, the seal moveable between a first position wherein the removable seal overlaps and engages the first membrane and hermetically isolates the first well and a second position wherein the removable seal is spaced from the membrane so as to allow the second substance to diffuse through the first membrane;
• and
• a non-porous second membrane extending over the second port, the second membrane preventing the first substance and the second substance from diffusing therethrough.