US 9,283,257 B2 · Compositions comprising solanum tuberosum for lowering cytosolic NADH level to mimic calorie restriction

Abstract

Embodiments herein relate to agents that mimic calorie restriction (CR) to extend life span, especially agents that lower the level of cytosolic NADH and increase the level of cytosolic NAD+ (relative to NADH) required to mimic calorie restriction that results in life span extension, at least in part by inhibition by NADH of SIR2, which is a key regulator of life span in both yeast and animals. NADH is a competitive inhibitor of SIR2, an NAD-requiring NAD-dependent histone deacetylase required for chromatin silencing and life-span extension. An overall reduction in NADH activates SIR2 and extends life span. The use of fluorescence measurements of NADH levels in a 96-well plate assay utilizing Baker's yeast (Saccharomyces cerevisiae) as a model to screen for agents and supplements that lower NADH levels is disclosed herein. A caloric restriction mimetic contains 1 part Mg2+ malate, 1 part turmeric and 98 parts dehydrated potato skins.

Claims16

Dependent claims

A nutritional supplement composition comprising:

a) at least one of dried and milled Irish potato (Solanum tuberosum) skins, dehydrated hot water extracts of whole Irish potatoes, dehydrated Irish potato skins, skins of sweet potato (Ipomea batas) or hulls of pigmented bean (Phaseolus vulgarus) seeds;
b) at least one of turmeric or curcumin;
c) at least one of Mg2+-malate or sodium malate;
and
d) one selected from the group consisting of oxaloacetic acid, Mg++-oxaloacetate or other pharmaceutically acceptable salts of oxaloacetic acid;
wherein at least one of the dried and milled Irish potato (Solanum tuberosum) skins, the dehydrated hot water extracts of whole Irish potatoes, the dehydrated potato skins, the skins of sweet potato (Ipomea batas), the hulls of pigmented bean (Phaseolus vulgarus) seeds, the turmeric, the curcumin, the Mg2+-malate, the sodium malate, the oxaloacetic acid, the Mg++-oxaloacetate or the other pharmaceutically acceptable salts of oxaloacetic acid is in an amount sufficient to reduce cytosolic NADH to levels that mimic calorie restriction.

3 more elements

Dependencies are inferred from the claim text.

Compositions comprising solanum tuberosum for lowering cytosolic NADH level to mimic calorie restriction

Life of this patent

Compositions comprising solanum tuberosum for lowering cytosolic NADH level to mimic calorie restriction

Life of this patent

Abstract

Claims16