Use of stem cell conditioned medium to induce ZO-1 proteins expression for skin regeneration, repair and firming
AssigneeGrowgene Biotech Inc.
InventorsPei-Chuan Chuang, Huei-Chun Liu
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Abstract
A use of a stem cell conditioned medium to induce ZO-1 proteins expression for skin regeneration, repair and finning is revealed herein. First, mesenchymal stem cells are cultured in a cell culture dish containing complete growth media, wherein the complete growth media include α-MEM, fetal bovine serum, and human-basic fibroblast growth factors. After mesenchymal stem cells are sub-cultured in the complete growth media for three times, a conditioned medium which can effectively increase the activation of tight junction protein Zonula occludens-1 (ZO-1) can be acquired from the basal medium.
Claims2
A method of firming skin using a stem cell conditioned medium, comprising:
- applying an effective amount of the stem cell conditioned medium to the skin of a subject in need thereof so as to induce ZO-1 protein expression thereby firming the skin;
- and wherein said stem cell conditioned medium is produced by:
- (a) culturing human Wharton's jelly mesenchymal stem cells in a cell culture dish containing a complete growth medium, wherein the complete growth medium includes α-MEM, fetal bovine serum, and human-basic fibroblast growth factors;
- and (b) sub-culturing the human Wharton's jelly mesenchymal stem cells in the complete growth medium at least three times and transferring the stem cells to a basal medium to obtain said stem cell conditioned medium, wherein the basal medium includes α-MEM and human-basic fibroblast growth factors.
Dependencies are inferred from the claim text.