US 9,282,746 B2Granted

Perhydrolase

AssigneeDanisco US Inc.

InventorsNeelam S. Amin, Matthew G. Boston, Richard R. Bott, Maguerite A. Cervin, Edward M. Concar, Marc E. Gustwiller, Brian E. Jones, Klaus Liebeton, Gregory S. Miracle, Hiroshi Oh, Ayrookaran J. Poulose, Sandra W. Ramer, Jeffrey J. Scheibel, Walter Weyler, Gregory M. Whited

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Abstract

The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.

Claims13

Dependent claims

A cleaning, bleaching or disinfecting composition, comprising an isolated perhydrolase, which is a homolog of M. smegmatis perhydrolase and is a SGNH-hydrolase family polypeptide, which comprises:

a) the motifs GDSL, GRTT, and GAND;
and
b) the amino acid residues corresponding to L6, W14, W34, L38, R56, D62, L74, L78, H81, P83, M90, K97, G110, L114, L135, F180, G205, S11, D192, and H195 of SEQ ID NO:
2;
wherein said perhydrolase exhibits:
i) perhydrolysis activity that is at least about 0.2 compared to the perhydrolysis activity exhibited by M. smegmatis perhydrolase;
and
ii) a perhydrolysis to hydrolysis ratio that is greater than 1;
wherein said perhydrolysis activity and said perhydrolysis to hydrolysis ratio is determined using the perhydrolysis and hydrolysis assays as described in Example 2.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/273,384Filed 8 May 2014 · continuation
Priority: 3 Dec 2003
Published: US 2014/0302003 A19 Oct 2014
Granted: 15 Mar 2016
Term ends: Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12N 9/52
Proteinases , e.g. Endopeptidases: derived from bacteria or Archaea
A01N 63/50
Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances…:…
C11D 3/38636
Preparations containing enzymes , e.g. protease or amylase: containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
C12N 9/16
Hydrolases: acting on ester bonds

Family

Application60/526,764Us provisional application · filed 3 Dec 2003
PCT/US2004/040438Parent of this continuation · filed 3 Dec 2004
US 2014/0302003 A1Pre-grant publication · published 9 Oct 2014
Application10/581,014Parent of this continuation
US 8,772,007 B2Parent of this continuation
US 2008/0145353 A1Published application
US 9,282,746 B2This patent

From the US filing history. Foreign family members aren’t listed.