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US 9,279,814 B2Granted
Solid state synthesis hydroxyl radicals for high throughput structure determination of proteins and nucleic acids by oxidative footprinting
AssigneeAlbert Einstein College of Medicine
InventorsMichael Brenowitz, Jorg Schlatterer
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Abstract
The present invention generally relates to an apparatus for structural mapping of a macromolecule comprising an amount of persulfide effective to generate hydroxyl radicals upon contact with an aqueous solution. The present invention further relates to methods for structural mapping a macromolecule in an aqueous solution and methods for structural mapping a plurality of macromolecules in parallel, wherein each macromolecule is in a separate aqueous solution.
Claims7
A method for structural mapping a macromolecule in an aqueous solution comprising:
- (a) contacting the aqueous solution containing the macromolecule with a persulfide of a persulfide-lined chamber such that hydroxyl radicals are generated;
- (b) incubating the aqueous solution containing the macromolecule such that the macromolecule is (i) cleaved by the hydroxyl radicals, thereby producing cleaved macromolecule fragments or (ii) oxidized by the hydroxyl radicals, thereby producing macromolecules of altered molecular weight;
- and (c) analyzing the cleaved macromolecule fragments or macromolecules of altered molecular weight produced in step (b) via a structural genomics method, thereby structural mapping the macromolecule.
Dependencies are inferred from the claim text.