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US 9,279,106 B2Granted
Immortalization of epithelial cells and methods of use
AssigneeGeorgetown University
InventorsRichard Schlegel, Xuefeng Liu
Life of this patent
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Abstract
The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.
Claims10
A method of continuously culturing non-keratinocyte epithelial cells, the method comprising
- a) culturing the non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium, and
- b) inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing,
- wherein the continuously cultured non-keratinocyte epithelial cells are not embryonic or adult stem cells and the continuously cultured non-keratinocyte epithelial cells become conditionally immortalized.
Dependencies are inferred from the claim text.