US 9,274,129 B2Granted

Methods and reagents for detecting bioactive lipids

AssigneeLpath, Inc.

InventorsRoger A. Sabbadini, William A. Garland, Genevieve Hansen

Life of this patent

Estimating the term…

Loading patent…

Abstract

Compositions and methods for producing monoclonal antibodies and their derivatives reactive against bioactive lipid targets are described. These compositions include derivatized lipids, each of which comprises a bioactive lipid that having a polar head group and at least one hydrocarbon chain (e.g., a lysolipid such as lysophosphatidic acid or sphingosine-1-phosphate) in which a carbon atom has been derivatized with a pendant reactive group; immunogens made by linking a derivatized lipid to a carrier moiety (e.g., a carrier protein, polyethylene glycol, colloidal gold, alginate, or a silicone bead); monoclonal antibodies and derivatives produced by immunizing an animal with such an immunogen; and therapeutic and diagnostic compositions containing such antibodies and antibody derivatives. Methods for making such derivatized lipids, immunogens, and monoclonal antibodies and derivatives, methods for detecting such antibodies once generated, and therapeutic and diagnostic methods for using such antibodies and derivatives, are also described.

Claims7

Dependent claims

A diagnostic reagent, comprising a solid support and a derivatized lipid covalently attached thereto either directly or indirectly, wherein the derivatized lipid is a derivative of a bioactive lysolipid, which lysolipid has a polar head group and a single hydrocarbon chain and is selected from the group consisting of:

(a) a lysolipid having a glycerol backbone with a polar head group at carbon sn-3 and a single hydrocarbon chain contained in a single fatty acid moiety attached at either carbon sn-1 or carbon sn-2, the carbon atom of the terminal methyl group of said hydrocarbon chain of the fatty acid having been derivatized with a sulfhydryl group by substitution of a hydrogen of the terminal methyl group with said sulfhydryl group prior to attachment to said solid support via said sulfhydryl group;
and
(b) a lysolipid having a sphingoid backbone with a polar head group and having the terminal methyl group of the single hydrocarbon chain of the sphingoid backbone of the lysolipid derivatized with a sulfhydryl group by substitution of a hydrogen of the terminal methyl group with said sulfhydryl group prior to attachment to said solid support via said sulfhydryl group and wherein the lysolipid is sphingolipid or a sphingolipid metabolite.

Dependencies are inferred from the claim text.

Facts

Application: 11/755,699Filed 30 May 2007 · us provisional application
Priority: 31 May 2006
Published: US 2008/0090303 A117 Apr 2008
Granted: 1 Mar 2016
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

G01N 33/92
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
C07K 16/18
Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies: against material from animals or humans
C07K 16/30
Against receptors, cell surface antigens or cell surface determinants: from tumour cells
G01N 33/6854
Involving proteins, peptides or amino acids: immunoglobulins

Family

Application60/810,185Us provisional application · filed 31 May 2006
Application60/835,569Us provisional application · filed 4 Aug 2006
Application60/923,644Us provisional application · filed 16 Apr 2007
US 2008/0090303 A1Pre-grant publication · published 17 Apr 2008
US 9,274,129 B2This patent

From the US filing history. Foreign family members aren’t listed.