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US 9,274,124 B2Granted
Mass spectrometric assays for peptides
AssigneeAnderson Forschung Group, Inc.
InventorNorman Leigh Anderson
Life of this patent
Estimating the term…
Abstract
Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry.
Claims24
A carrier stable isotope standard (carrierSIS) complex comprising:
- a carrier having at least one carrier linkage site and at least one extended stable isotope standard (SIS) peptide having a peptide linkage site, wherein said SIS peptide is isotope labeled in one or more amino acids of said peptide;
- wherein said carrier and said peptide are bound through a covalent linkage between said peptide linkage site and said carrier linkage site, wherein the linkage between said carrier linkage site and said peptide linkage site is not a proteolytic enzyme cleavage site,
- wherein said SIS peptide can be liberated from said carrier by action of a proteolytic process yielding at least one free SIS peptide that is chemically identical to the natural peptide for which said SIS peptide is a standard except for said isotope labeling, and
- wherein more than 90% of the SIS peptides bound to said carrier have the correct sequence compared to the natural peptide for which said SIS peptides are a standard.
1 more element
A carrier stable isotope standard (carrierSIS) complex comprising:
- a carrier having at least one carrier linkage site and at least one extended stable isotope standard (SIS) peptide having a peptide linkage site;
- wherein said carrier and said peptide are bound through a covalent linkage between said peptide linkage site and said carrier linkage site, wherein the linkage between said carrier linkage site and said peptide linkage site is not a proteolytic enzyme cleavage site,
- wherein said SIS peptide can be liberated from said carrier by action of a proteolytic process yielding at least one free SIS peptide,
- wherein more than 90% of the SIS peptides bound to said carrier have the correct sequence compared to the natural peptide for which said SIS peptides are a standard,
- and wherein the carrier is a magnetic particle.
2 more elements
A carrier stable isotope standard (carrierSIS) complex comprising:
- a carrier having at least one carrier linkage site and at least one extended stable isotope standard (SIS) peptide having a peptide linkage site;
- wherein said carrier and said peptide are bound through a covalent linkage between said peptide linkage site and said carrier linkage site, wherein the linkage between said carrier linkage site and said peptide linkage site is not a proteolytic enzyme cleavage site,
- wherein said SIS peptide can be liberated from said carrier by action of a proteolytic process yielding at least one free SIS peptide,
- wherein more than 90% of the SIS peptides bound to said carrier have the correct sequence compared to the natural peptide for which said SIS peptides are a standard,
- and wherein the at least one extended SIS peptide is an extended polySIS peptide comprising different SISpeptide sequences in different relative proportions.
2 more elements
An assay kit, comprising:
- (a) a carrierSIS complex comprising:
- a carrier having at least one carrier linkage site and at least one extended SIS peptide having a peptide linkage site;
- wherein said carrier and said peptide are bound through a covalent linkage between said peptide linkage site and said carrier linkage site, wherein the linkage between said carrier linkage site and said peptide linkage site is not a proteolytic enzyme cleavage site,
- wherein said SIS peptide can be liberated from said carrier by action of a proteolytic process yielding at least one free SIS peptide, and
- (b) antibodies specific to said at least one free SIS peptide.
2 more elements
Dependencies are inferred from the claim text.