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US 9,273,308 B2Granted
Selection of compartmentalized screening method
AssigneeRaindance Technologies, Inc.
InventorsDarren R. Link, Michael Weiner, David Marran, Jonathan M. Rothberg
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Abstract
The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
Claims23
A method for analyzing an amplification reaction product, the method comprising:
- providing a plurality of droplets separated by an oil in a reservoir that is external to a microfluidic device, wherein a plurality of the droplets comprise nucleic acid molecules and reagents in an aqueous fluid for a nucleic acid amplification reaction;
- conducting the nucleic acid amplification reaction simultaneously in the droplets while the droplets are in the reservoir;
- subsequent to the conducting step, moving the droplets from the reservoir into a channel of the microfluidic device;
- and
- flowing the droplets in the microfluidic device past an optical detector, wherein an optical signal in at least one of the droplets is detected, the optical signal indicating presence of a nucleic acid amplification product in at least one of the at least one droplets.
2 more elements
Dependencies are inferred from the claim text.