Enzymatic alkene cleavage
AssigneeUniversitat Graz
InventorsAashrita Rajagopalan, Wolfgang Kroutil, Markus Schober
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Abstract
The invention relates to an enzyme that comprises or includes a sequence according to SEQ. ID No. 1 or SEQ. ID No. 2, to a method for the production thereof, and to the use thereof as a catalyst in the oxidative cleavage of vinyl aromatics.
Claims13
A method of cleaving vinyl aromatics, the method comprising cleaving a vinyl aromatic in the presence of oxygen using an enzyme as a catalyst, wherein the enzyme comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1 or SEQ ID NO:
- 2.
A method of preparing an isolated enzyme comprising the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, the method comprising steps of:
- (i) culturing a strain of Trametes hirsuta;
- and
- (ii) recovering the enzyme from a cell-free extract of the culture of step (i).
A method of recombinantly producing an isolated enzyme comprising the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, the method comprising steps of:
- (i) transforming a nucleic acid comprising the nucleotide sequence of SEQ ID NO:
- 3 or SEQ ID NO:
- 4 into cells suitable for expressing the enzyme comprising the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2;
- (ii) incubating the cells under conditions suitable for expressing the enzyme in a culture broth;
- and
- (iii) isolating the expressed enzyme from the culture broth.
Dependencies are inferred from the claim text.