Heterologous expression of cellular adhesion molecules
AssigneeUniversity of Wyoming
InventorsStephen K. Herbert, Levi G. Lowder
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Abstract
Methods for the production of unicellular photosynthetic organisms capable of producing cell adhesion proteins are disclosed. DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular photosynthetic organisms for the expression of cell adhesion proteins are also disclosed.
Claims8
A transgenic unicellular photosynthetic organism chosen from Chlamydomonas spp, and Dunaliella spp., having a DNA construct stably integrated into the unicellular photosynthetic organism's genome under conditions suitable for expression of said DNA construct in an extracellular matrix, cell wall, plasma membrane or outer membrane of said unicellular photosynthetic organism, wherein said DNA construct comprises a promoter, wherein said promoter is operably linked to a cell adhesion protein coding sequence, wherein said cell adhesion protein coding sequence is chosen from an Algal-CAM protein, an ISG protein, a V1 protein, a V2 protein, a cadherin protein and a claudin protein wherein said cell adhesion protein coding sequence is expressed as a heterologous protein in the extracellular matrix, cell wall, plasma membrane or outer membrane of the organism, and wherein the expressed cell adhesion protein induces the unicellular photosynthetic organism to flocculate by adhering to one or more unicellular photosynthetic organisms.
Dependencies are inferred from the claim text.