Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array
AssigneeCustomArray, Inc.
InventorsKarl Maurer, John J Cooper
Life of this patent
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Abstract
There is disclosed an electrochemical deblocking solution for use on an electrode microarray. There is further disclosed a method for electrochemical synthesis on an electrode array using the electrochemical deblocking solution. The solution and method are for removing acid-labile protecting groups for synthesis of oligonucleotides, peptides, small molecules, or polymers on a microarray of electrodes while substantially improving isolation of deblocking to active electrodes. The method comprises applying a voltage or a current to at least one electrode of an array of electrodes. The array of electrodes is covered by the electrochemical deblocking solution.
Claims20
An electrochemical deblocking solution comprising:
- an organic base including N,N-diisopropylethylamine, a benzoquinone and a hydroquinone;
- tetrabutylammonium hexafluorophosphate as an organic salt;
- and
- a solvent including acetonitrile and an alcohol.
An electrochemical deblocking solution comprising:
- an organic base including a lutidine, a benzoquinone and a hydroquinone;
- tetraethylammonium p-toluenesulfonate as an organic salt;
- and
- a solvent selected from the group consisting of two or more of an alcohol, acetonitrile and dichloromethane.
An electrochemical deblocking solution for use on an electrode microarray comprising:
- approximately 0.01 to 200 mM of a pyridine derivative or isomer thereof, where the pyridine derivative or isomer thereof is not bound to the electrode microarray;
- approximately 0.1 to 100 mM 2,5-di-tert-butyl benzoquinone;
- approximately 0.1 to 100 mM 2,5-di-tert-butyl hydroquinone;
- approximately 0.1 mM to 5 M tetraethylammonium p-toluenesulfonate;
- and
- a solvent having approximately 10% to 99% acetonitrile and approximately 1% to 90% methanol.
Dependencies are inferred from the claim text.