Terpene synthases from Santalum
AssigneesThe University of British Columbia, The University of Western Australia, Forest Products Commission
InventorsKatherine Zulak, Christopher Jones, Jessie Moniodis, Joerg Bohlmann
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Abstract
An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.
Claims26
A host cell, comprising a cDNA molecule that encodes a Santalum species terpene synthase that comprises:
- (i) the sequence of amino acid resides set forth in SEQ ID NO:2, 4 or 6 or a catalytically active fragment thereof;
- or
- (ii) a sequence of amino acid residues that has at least 95% or greater sequence identity to the sequence of amino acid residues set forth in SEQ ID NO:
- 2, or a catalytically active fragment thereof, wherein:
- the cDNA molecule is heterologous to the cell;
- and the encoded synthase catalyzes the production of an α-santalene, α-trans-bergamotene, epi-β-santalene and β-santalene concurrently.
A vector, comprising a cDNA molecule that encodes a Santalum species terpene synthase that comprises:
- (i) the sequence of amino acid resides set forth in SEQ ID NO:2, 4 or 6 or a catalytically active fragment thereof;
- or
- (ii) a sequence of amino acid residues that has at least 95% or greater sequence identity to the sequence of amino acid residues set forth in SEQ ID NO:
- 2, or a catalytically active fragment thereof,
- wherein the encoded synthase catalyzes the production of an α-santalene, α-trans-bergamotene, epi-β-santalene and β-santalene concurrently.
A method of making a terpene, comprising:
- a) contacting an acyclic pyrophosphate terpene precursor with the terpene synthase encoded by a nucleic acid cDNA molecule to produce a terpene;
- and, b) optionally, isolating the terpene produced in step (a), wherein:
- the cDNA molecule that encodes a Santalum species terpene synthase comprises:
- (i) the sequence of amino acid resides set forth in SEQ ID NO:2, 4 or 6 or a catalytically active fragment thereof;
- or
- (ii) a sequence of amino acid residues that has at least 95% or greater sequence identity to the sequence of amino acid residues set forth in SEQ ID NO:
- 2, or a catalytically active fragment thereof;
- the encoded synthase catalyzes the production of an α-santalene, α-transbergamotene, epi-β-santalene and β-santalene concurrently;
- and
- if the terpene is produced in a host cell, the cDNA molecule is heterologous to the host cell.
Dependencies are inferred from the claim text.