Apparatus and methods relating to collecting and processing human biological material containing adipose

An apparatus useful for collection of human biological material and post-collection processing of collected material, the apparatus being orientable in a collection orientation, as oriented in the collection orientation the apparatus comprising:

• a filter;
• a container having an internal containment volume, the internal containment volume comprising:
• a tissue retention volume and a filtrate volume separated by the filter;
• a collection volume within the filtrate volume, the collection volume having a bottom elevation corresponding to a bottom elevation of the filtrate volume and a top elevation that is lower than the bottom elevation of the tissue retention volume;
• a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located above the collection volume;
• an inlet port in fluid communication with the tissue retention volume and configured for introducing human biological material comprising adipose directly into the tissue retention volume;
• a suction port in fluid communication with the filtrate volume and providing access to the filtrate volume for suctioning from the filtrate volume components passing through the filter from the tissue retention volume to the filtrate volume;
• a mixing device disposed at least in part within the tissue retention volume for mixing contents within the tissue retention volume, the mixing device comprising a rotatable shaft extending from outside of the internal containment volume to inside of the internal containment volume;
• and
• a lumen extending through the shaft and having a proximal end located outside of the internal containment volume and a distal end located within the internal containment volume, thereby providing access from outside of the internal containment volume to inside of the internal containment volume.