Method for developing natural killer cells from stem cells
OwnerDan S. Kaufman, David A. Knorrinventors
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Abstract
A method for producing NK cells from pluripotent stem cells, which includes culturing pluripotent stem cells in a first serum-free medium, aggregating the undifferentiated stem cells to form embryoid bodies, which are cultured to produce hematopoietic precursor cells, and culturing the precursor cells in a serum-free medium to produce the NK cells. Methods for using such NK cells, e.g., in the treatment of cancer and infectious disease are also provided.
Claims17
A method for producing natural killer cells from undifferentiated stem cells, the method comprising:
- placing undifferentiated stem cells in a serum-free medium;
- aggregating the undifferentiated stem cells in the serum-free medium and forming spin embryoid bodies by spin aggregation;
- culturing the spin embryoid bodies in the serum-free medium for inducing production of precursor cells from the spin embryoid bodies;
- culturing the precursor cells in a second serum-free medium and in the absence of exogenous stromal cells to produce the natural killer cells from the precursor cells;
- and
- co-culturing the natural killer cells with inactivated artificial antigen presenting cells (aAPCs).
A method for producing natural killer cells from pluripotent stem cells, the method comprising:
- (a) aggregating the pluripotent stem cells in a first serum-free medium, thereby forming embryoid bodies;
- (b) culturing the embryoid bodies in a second serum-free medium, thereby producing hematopoietic progenitor cells;
- and
- (c) culturing the hematopoietic progenitor cells in a third serum-free medium comprising interleukin 3 (IL-3), interleukin 7 (IL-7), interleukin 15 (IL-15), SCF, and Fms-related tyrosine kinase 3 ligand (FLT3L) and in the absence of exogenous stroma or stromal cells, thereby producing natural killer cells;
- and
- (d) co-culturing the natural killer cells with inactivated artificial antigen presenting cell (aAPCs).
Dependencies are inferred from the claim text.