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AssigneeSolazyme, Inc.
InventorsScott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
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Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
A recombinant eukaryotic host cell comprising an exogenous nucleic acid encoding a THIC enzyme, wherein the recombinant eukaryotic host cell expresses the nucleic acid and is capable of growing in a thiamine-free culture medium.
A method of cultivating a recombinant eukaryotic host cell, the host cell comprising an exogenous nucleic acid encoding a THIC enzyme, wherein the recombinant eukaryotic host cell expresses the nucleic acid and is capable of growing in a thiamine-free culture medium, the method comprising the steps of:
Dependencies are inferred from the claim text.