Polynucleotide encoding PPAT derived from jatropha and use thereof
AssigneesSumitomo Electric Industries, Ltd., Osaka University
InventorsKiichi Fukui, Joyce Cartagena, Naoki Wada, Tsutomu Kohinata, Yasuhisa Yushio, Naoki Ikeguchi, Satoshi Tabata
Life of this patent
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Abstract
A PPAT polypeptide of SEQ ID NO: 1 derived from Jatropha, a PPAT polynucleotide of SEQ ID NO: 2 and so on were found. By transforming Jatropha with these PPAT polynucleotides, it is possible to overexpress the PPAT polypeptide in comparison with a wild type, and biosynthesis of coenzyme A is promoted by these polypeptides, the metabolic function and viability of the transformed Jatropha are enhanced, and for example, stress resistance can be significantly improved.
Claims10
A vector comprising:
- (a) a cDNA comprising the polynucleotide represented by SEQ ID NO:
- 2 or
- (b) a polynucleotide encoding a phosphopantetheine adenylyltransferase (PPAT) polypeptide having a homology of 95% or higher with the amino acid sequence of SEQ ID NO:
- 1, wherein said polynucleotide is operably linked to a heterologous regulatory element.
A cDNA comprising the polynucleotide represented by SEQ ID NO:
- 2.
An isolated PPAT polypeptide other than the polypeptide of SEQ ID NO:
- 1, wherein said polypeptide has an amino acid sequence having a homology of 95% or higher with the amino acid sequence of SEQ ID NO:
- 1, and wherein the polypeptide catalyzes an exchange reaction between 4′-phosphopantetheine and dephospho-coenzyme A.
A method of producing a stress resistant Jatropha plant, comprising:
- producing an isolated polynucleotide selected from the following polynucleotides:
- (a) a cDNA comprising the polynucleotide represented by SEQ ID NO:
- 2;
- or
- (b) a polynucleotide encoding a PPAT polypeptide having a homology of 95% or higher with the amino acid sequence of SEQ ID NO:
- 1;
- producing a Jatropha plant transformation vector comprising said isolated polynucleotide;
- and
- transforming a Jatropha plant with said vector.
Dependencies are inferred from the claim text.