Back to results
US 9,255,261 B2Granted
Ultrapure hypoallergenic solutions of sacrosidase
AssigneeQOL Medical LLC
InventorsDayton T. Reardan, Christopher Seekamp
Life of this patent
Estimating the term…
Abstract
One aspect provides an ultrapure, hypoallergenic sacrosidase. Another aspect provides a solution of sacrosidase in about 1:1 glycerol/water having an enzymatic activity of at least about 7500 IU/mL and a residual papain concentration that does not include an allergic reaction in a human patient when given a dose of about 2.0 mL/day.
Claims5
Hypoallergenic sacrosidase prepared by a process comprising:
- (a) digesting an aqueous suspension of saccharomyces cerevisiae at a pH of about 7.0 with an amount of papain effective to cleave non-sacrosidase proteins in said suspension while not cleaving sacrosidase in said suspension;
- (b) acidifying and filtering the digested suspension to remove cell debris yield an acidified filtrate;
- (c) treating the acidified filtrate with one or more decolorizing agents to yield a decolorized suspension;
- (d) filtering and concentrating the decolorized suspension to yield a concentrated sacrosidase solution;
- (e) diafiltering the concentrated sacrosidase solution to yield a solution of-hypoallergenic sacrosidase, wherein the primary structure of the sacrosidase is a 513 amino acid polypeptide that is glycosylated, and wherein the solution has a sacrosidase activity of at least about 22,000 IU/mL in water at a pH of about 4.6 ±0.1 and has a residual papain level of less than about 50 ppb (50 nanograms/mL) papain.
2 more elements
Dependencies are inferred from the claim text.