Recombinant microorganisms having phosphoketolase activity and diminished phosphofructokinase activity and/or glucose-6 phosphate dehydrogenase activity
AssigneeScientist of Fortune, S.A.
InventorPhilippe Marliere
Life of this patent
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Abstract
Described are recombinant microorganisms characterized by having phosphoketolase activity, having a diminished or inactivated Embden-Meyerhof-Parnas pathway (EMPP) by inactivation of the gene(s) encoding phosphofructokinase or by reducing phosphofructokinase activity as compared to a non-modified microorganism and having a diminished or inactivated oxidative branch of the pentose phosphate pathway (PPP) by inactivation of the gene(s) encoding glucose-6-phosphate dehydrogenase or by reducing glucose-6-phosphate dehydrogenase activity as compared to a non-modified microorganism. These microorganisms can be used for the production of useful metabolites such as acetone, isobutene or propene.
Claims68
A genetically modified prokaryotic microorganism comprising:
- (a) increased phosphoketolase activity as compared to a non-genetically modified microorganism;
- and
- (b) (i) diminished or inactivated phosphofructokinase activity as compared to a non-genetically modified microorganism;
- or
- (ii) not possessing phosphofructokinase activity;
- and
- (c) (i) diminished or inactivated glucose-6-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism;
- or
- (ii) not possessing glucose-6-phosphate dehydrogenase activity;
- and either
- (d) (i) reduced glyceraldehyde 3-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism;
- or
- (ii) increased fructose-1,6-bisphosphate phosphatase activity when grown on glucose as compared to a non-genetically modified microorganism.
A genetically modified prokaryotic microorganism comprising:
- (a) increased phosphoketolase activity as compared to a non-genetically modified microorganism;
- and
- (b) (i) diminished or inactivated phosphofructokinase activity as compared to a non-genetically modified microorganism;
- or
- (ii) not possessing phosphofructokinase activity;
- and
- (c) (i) diminished or inactivated glucose-6-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism;
- or
- (ii) not possessing glucose-6-phosphate dehydrogenase activity;
- and either
- (d) (i) reduced glyceraldehyde 3-phosphate dehydrogenase activity as compared to a non-genetically modified microorganism;
- or
- (ii) increased fructose-1,6-bisphosphate phosphatase activity when grown on glucose as compared to a non-genetically-modified microorganism;
- wherein the genetically modified prokaryotic microorganism is capable of converting acetyl-CoA into acetone, isobutene, or propene.
Dependencies are inferred from the claim text.