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US 9,243,288 B2Granted
Cartridge with lysis chamber and droplet generator
AssigneeBio-Rad Laboratories, Inc.
InventorsKevin Dean Ness, Samuel Burd, Benjamin Joseph Hindson, Phillip Belgrader, Billy W. Colston, Jr.
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Abstract
Systems, including apparatus, methods, compositions, kits, and software, for preparing, reacting, detecting, and/or analyzing samples in droplet-based assay systems, among others. The disclosure emphasizes, but is not limited to, a disposable cartridge with lysis chamber and droplet chamber, particularly for use in droplet-based assays.
Claims33
A method of nucleic acid amplification, comprising:
- purifying a fluid sample in a portion of a disposable, single-use cartridge;
- lysing the sample in a portion of the cartridge;
- combining the purified, lysed sample with a reagent mixture in a portion of the cartridge;
- driving flow of at least one carrier fluid stream and a sample stream to a channel intersection at which droplets containing the sample are generated to form an emulsion, wherein the sample stream includes the purified, lysed sample combined with the reagent mixture;
- and
- cycling the emulsion thermally in a thermocycling instrument to which the emulsion is transferred from the cartridge,
- wherein the step of combining includes a step of advancing a first plunger that is operatively connected to a first reservoir containing one of the sample and the reagent mixture, while retracting a second plunger that is operatively connected to a second reservoir containing the other of the sample and the reagent mixture, to move fluid out of the first reservoir and into the second reservoir.
4 more elements
Dependencies are inferred from the claim text.