Filamentous fungal mutants with improved homologous recombination efficiency
AssigneeDsm IP Assets B.V.
InventorsPetrus Jacobus Theodorus Dekker, Marco Alexander van den Berg
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Abstract
The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.
Claims13
A mutant of a parent filamentous fungus, the parent filamentous fungus having a preference for non-homologous recombination (NHR), wherein the ratio of NHR/HR is decreased in the mutant as compared to said ratio in said parent filamentous fungus measured under the same conditions and wherein the mutant is deficient in a gene encoding a component involved in NHR, or has a decreased level of a component involved in NHR, wherein the mutant is, optionally inducibly, deficient in at least one filamentous fungal homologue of yeast KU80, yeast KU70, or both, or has, optionally inducibly, a decreased amount of at least one of the proteins encoded by these genes, and wherein the parent filamentous fungus belongs to the genus Aspergillus.
Dependencies are inferred from the claim text.