Method for producing natively folded proteins in a prokaryotic host
AssigneeUniveristy of Oulu
InventorLloyd Ruddock
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Abstract
The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.
Claims13
A method for producing a eukaryotic recombinant protein of interest containing one or more disulfide bonds in a prokaryotic host, said method comprising expressing in the cytoplasm of the prokaryotic host cell
- a nucleotide sequence encoding the eukaryotic protein of interest;
- and
- a nucleotide sequence encoding sequence of sulfhydryl oxidase catalyzing reaction dithiol+O2→2disulfides+2H2O;
- whereby said eukaryotic recombinant protein of interest is formed in a soluble form and containing natively folded disulfide bonds in the cytoplasm of said host cell.
Dependencies are inferred from the claim text.