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US 9,238,800 B2Granted
Avian adenoassociated virus and uses thereof
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsIoannis Bossis, John A. Chiorini
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Abstract
The present invention provides an Avian adeno-associated vims (AAAV) vims and vectors and particles derived therefrom. In addition, the present invention provides methods of delivering a nucleic acid to a cell using the AAAV vectors and particles. Methods of isolating the AAAV are provided.
Claims7
A method of producing AAAV virus particles comprising
- a) transducing a cell with a vector system comprising at least one nucleic acid molecule selected from the group consisting of:
- a nucleic acid molecule comprising a pair of inverted terminal repeats (ITRs), each of which is capable of forming a T-shaped hairpin structure, and a heterologous nucleic acid sequence between the inverted terminal repeats, wherein at least one inverted terminal repeat comprises SEQ ID NO:20 and SEQ ID NO:21, and wherein at least one ITR can be used as an origin of replication;
- and,
- a recombinant nucleic acid molecule that encodes a protein comprising SEQ ID NO:11, SEQ ID NO:13 or SEQ ID NO:15, wherein the recombinant nucleic acid molecule is a plasmid, a yeast artificial chromosome or a non-AAV viral vector;
- and
- b) isolating AAAV virus particles.
3 more elements
Dependencies are inferred from the claim text.