Method for collecting a desired blood component and performing a photopheresis treatment

A disposable closed-circuit kit for single use in a reusable tower system, the reusable tower system including at least one pump and at least one processor, the at least one processor being operably coupled to the at least one pump and the kit for continuously separating at least one first component from a biological fluid and subjecting the at least one first component to at least one therapeutic application, the kit comprising:

• a first needle adapter for obtaining the biological fluid from a subject;
• a cassette for operably coupling to the at least one pump to direct a flow of at least one fluid through the kit;
• a single centrifuge bowl for operably coupling to the at least one processor, the centrifuge bowl comprising:
• an inlet port to control a rate of inflow of the biological fluid into the centrifuge bowl;
• a first outlet port to control a first rate of withdrawal of the at least one first component of the biological fluid from the centrifuge bowl;
• and
• a second outlet port to control a second rate of withdrawal of at least one second component of the biological fluid from the centrifuge bowl, such that the at least one first component of the biological fluid is at least one of accumulated in and withdrawn from the centrifuge bowl independently from the at least one second component of the biological fluid, thereby continuously separating the at least one first component from the biological fluid;
• a collection bag for receiving the at least one second component from the centrifuge bowl;
• a treatment bag for receiving the at least one first component from the centrifuge bowl;
• an irradiation chamber comprising two or more fluid channels for receiving and exposing the at least one first component and a photoactive agent to electromagnetic radiation, thereby subjecting the at least one first component to the at least one therapeutic application;
• a second needle adapter for returning at least one treated component of the biological fluid to the subject;
• and
• tubing operably coupled to the cassette for providing a closed circuit of sealed passageways, thereby directing a flow of at least:
• saline to the cassette;
• anticoagulant to the cassette;
• the biological fluid from the first needle adapter and to the cassette;
• the at least one second component to the collection bag;
• the at least one first component to the treatment bag;
• and
• the at least one treated component from the irradiation chamber and to the second needle adapter.