Methods and compositions for regulating RNA import into mitochondria
AssigneeThe Regents of the University of California
InventorsMichael A. Teitell, Carla M. Koehler, Geng Wang
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Abstract
Disclosed herein are methods of manipulating the processing, targeting, and/or internalization of a nucleic acid molecule. Also disclosed are recombinant and/or isolated nucleic acid molecules having a first nucleic acid sequence, which is a wild-type sequence or an altered sequence, directly or indirectly linked to a second nucleic acid sequence which is a mitochondria localization sequence, an RNA import sequence, or a combination thereof, and methods of using thereof.
Claims11
A method of manipulating the processing, targeting, and/or internalization of a nucleic acid molecule which comprises one or more of the following steps
- providing an altered tRNA aminoacyl stem that is directly or indirectly linked to the nucleic acid molecule to thereby enable the nucleic acid molecule to escape the nucleus of a cell;
- providing a mitochondria localization sequence that is directly or indirectly linked to the nucleic acid molecule to thereby cause the nucleic acid molecule to localize in the proximity of a mitochondrion;
- and
- providing an RNA import sequence that is directly or indirectly linked to the nucleic acid molecule to thereby cause the nucleic acid molecule to be internalized by a mitochondrion, and
- wherein the RNA import sequence is selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:38, SEQ ID NO:39, sequences which are about 15-30 nucleotides long and comprise SEQ ID NO:39, and sequences complementary thereto.
Dependencies are inferred from the claim text.