Sample detection method by thin-layer chromatography, thin-layer chromatography plate, and method for producing same

A thin-layer chromatography plate that has a first separating agent layer, whose direction of development is a first direction, and, in a second direction that is orthogonal to the first direction, a second separating agent layer adjacent to the first separating agent layer, wherein the first separating agent layer is formed by a first separating agent, which has an optical response to ultraviolet light or a coloring agent;

• the second separating agent layer is formed by a second separating agent, which has an optical response that is different from that of the first separating agent;
• and when a spot has been developed in a first direction in the first separating agent layer, a ratio of the spot migration distance W2, in cm, in a second direction in the first separating agent layer to the spot migration distance WI, in cm, in the first direction in the first separating agent layer is not more than +/−0.5,
• the first separating agent is a separating agent for optical isomers, and
• the separating agent for optical isomers contains a polysaccharide and an aromatic ester group, aromatic carbamoyl group, or aromatic ether group substituted for all or a portion of the hydroxyl groups or amino groups in the polysaccharide,
• the first separating agent layer is formed by coating a support with a first slurry containing the first separating agent and a coating solvent,
• the second separating agent layer is formed by coating the support with a second slurry containing the second separating agent and a coating solvent, and gypsum and an organic binder are used in combination as the binder in both the first and second slurries,
• the gypsum content in the first slurry, expressed as per 100 mass parts of the first separating agent, is 5 to 30 mass parts and the content of the organic binder in the first slurry, expressed as per i00 mass parts of the first separating agent, is 0.5 to 10 mass parts, and
• the gypsum content in the second slurry, expressed as per 100 mass parts of the second separating agent, is 1 to 30 mass parts and the content of the organic binder in the second slurry, expressed as per 100 mass parts of the second separating agent, is 0.5 to 10 mass parts.

A method of producing a thin-layer chromatography plate that has a first separating agent layer and a second separating agent layer adjacent thereto,

• the method comprising:
• a step of forming a first separating agent layer by coating a support with a first slurry that contains a coating solvent and a first separating agent having an optical response to ultraviolet light or a coloring agent;
• and
• a step of forming a second separating agent layer adjacent to the first separating agent layer, by coating the support with a second slurry that contains a coating solvent and a second separating agent having an optical response that is different from that of the first separating agent,
• wherein
• the same type of coating solvent is used for the first and second slurries,
• the first separating agent is a separating agent for optical isomers, and
• the separating agent for optical isomers contains a polysaccharide derivative comprising a polysaccharide and an aromatic ester group, aromatic carbamoyl group, or aromatic ether group substituted for all or a portion of the hydroxyl groups or amino groups in the polysaccharide,
• gypsum and an organic binder are used in combination as the binder in both the first and second slurries,
• the gypsum content in the first slurry, expressed as per 100 mass parts of the first separating agent, is 5 to 30 mass parts and the content of the organic binder in the first slurry, expressed as per 100 mass parts of the first separating agent, is 0.5 to 10 mass parts, and
• the gypsum content in the second slurry, expressed as per 100 mass parts of the second separating agent, is 1 to 30 mass parts and the content of the organic binder in the second slurry, expressed as per 100 mass parts of the second separating agent, is 0.5 to 10 mass parts.