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US 9,234,223 B2Granted
Method for producing L-cysteine
AssigneeAjinomoto Co., Inc.
InventorsShunsuke Yamazaki, Gen Nonaka, Kazuhiro Takumi
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Abstract
A method for producing L-cysteine and the like is provided by developing a novel technique for improving bacterial L-cysteine-producing ability. The method includes the steps of culturing a bacterium belonging to the genus Escherichia which has L-cysteine-producing ability, and in which expression of a gene involved in sulfite reduction is enhanced, in a medium containing thiosulfate, and collecting L-cysteine, a related substance thereof, or a mixture thereof which accumulate in the medium.
Claims5
A method for producing L-cysteine, a related substance thereof, or a mixture thereof, which comprises:
- A) culturing a bacterium belonging to the genus Escherichia in a medium comprising thiosulfate, which bacterium has L-cysteine-producing ability and is modified so that expression of a gene involved in sulfite reduction is increased by a method selected from the group consisting of increasing copy number of the gene involved in sulfite reduction, modifying an expression control sequence of the gene, and combinations thereof;
- and
- B) collecting L-cysteine, a related substance thereof, or a mixture thereof from the medium,
- wherein the gene involved in sulfite reduction is a cysG gene, and
- wherein the cysG gene comprises a DNA selected from the group consisting of:
- (a) a DNA comprising the nucleotide sequence of SEQ ID NO:
- 47 or 49,
- (b) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 48 or 50, and
- (c) a DNA encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 48 or 50, but which includes one to 10 amino acid substitutions, deletions, insertions, or additions.
8 more elements
Dependencies are inferred from the claim text.