Pest resistant plants
AssigneeKeygene N.V.
InventorsRobert Cornelis Schuurink, Michael Albertus Haring, Petronella Martina Bleeker
Life of this patent
Estimating the term…
Abstract
An isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising the nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves, are provided. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
Claims23
A method for preparing 7-epizingiberene and/or R-curcumene comprising:
- a) transforming a host cell with a recombinant nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence at least 99% identical to the full length of SEQ ID NO:
- 1, wherein in the transformed host cell the nucleic acid sequence encoding the polypeptide is operably linked to an active promoter;
- and
- b) culturing said host cell under conditions permitting production of 7-epizingiberene and/or R-curcumene.
A method for producing 7-epizingiberene from zFPP in a host cell, comprising:
- a) introducing into said host cell a first nucleic acid sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:6 or an amino acid sequence at least 95% identical to the full length of SEQ ID NO:6 over the entire length, and a second nucleic acid sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence at least 99% identical to the full length of SEQ ID NO:1, wherein in the host cell obtained then first nucleic acid sequence and second nucleic acid sequence are operably linked to an active promoter;
- b) culturing the host cell in suitable conditions for the expression of said first and said second nucleic acid sequences;
- and,
- c) collecting the 7-epizingiberene contained in said cell and/or in a culture medium thereof.
A method for producing a transgenic plant having enhanced insect pest resistance compared to a non-transgenic control plant, said method comprising the steps of:
- (a) transforming a plant or plant cell with a nucleic acid molecule encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence at least 99% identical to the full length of SEQ ID NO:1, operably linked to a promoter active in plant cells, and
- (b) regenerating a plant.
A method for preparing 7-epizingiberene and/or R-curcumene comprising the steps of:
- a) culturing a host cell transformed with a recombinant nucleic acid molecule comprising a nucleic acid sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence at least 99% identical to the full length of SEQ ID NO:
- 1, wherein in the host cell the nucleic acid sequence encoding the polypeptide is operably linked to an active promoter, under conditions permitting production of 7-epizingiberene and/or R-curcumene.
A method for preparing 7-epizingiberene and/or R-curcumene comprising the steps of:
- a) heterologously expressing in a host cell a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence at least 99% identical to the full length of SEQ ID NO:
- 1, under conditions permitting production of 7-epizingiberene.
Dependencies are inferred from the claim text.