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US 9,234,178 B2Granted
Differentiation of human pluripotent stem cells
AssigneeJanssen Biotech, Inc.
InventorsAlireza Rezania, Benjamin Fryer
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Abstract
The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependant kinase inhibitor to cause an increase in expression of MAFA.
Claims2
A method for increasing the expression of insulin and MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage, the method comprising the steps of:
- a. sequentially differentiating human pluripotent stem cells to obtain cells expressing markers characteristic of the pancreatic endocrine lineage;
- b. culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising an added amount of a cyclin-dependant kinase inhibitor to cause an increase in expression of insulin and MAFA as compared to cells expressing markers characteristic of the pancreatic endocrine lineage that are not cultured in medium comprising the added cyclin-dependent kinase inhibitor;
- wherein the cyclin-dependent kinase inhibitor is Ethyl-(6-hydroxy-4-phenylbenzo[4,5]furo[2,3-b])pyridine-3-carboxylate.
Dependencies are inferred from the claim text.