Method for purifying bee venom on mass scale
AssigneeRepublic of Korea (Management: Rural Development Administration)
InventorsSang Mi Han, Kwang Gill Lee, Kwan Kyu Park
Life of this patent
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Abstract
Disclosed is a method for the purification of bee venom on a mass scale. It comprises dissolving crude bee venom in a solvent to form a bee venom solution; applying an adsorbent to the bee venom solution to form a mixture; and removing the adsorbent through filtration to afford a pure bee venom solution. By the method of the present invention, crude bee venom collected from bee farms can be purified to a purity of 99% or higher on a mass scale, without inducing a change in the composition thereof, and thus the purified bee venom can be used as a material for medicines, cosmetics and foods.
Claims1
A method for producing purified bee venom on a mass scale, comprising:
- dissolving crude bee venom in water to form a bee venom solution, wherein the crude bee venom is present in the solution in an amount of 0.02-0.1 wt %;
- filtering the bee venom solution through a cellulose filter paper having a particle size of 20-30 μm under reduced pressure to remove non-water-soluble impurities;
- applying an absorbent to the filtered bee venom solution at a level of 0.1 to 10 g per liter of the bee venom solution to form a mixture, wherein the absorbent is a primary and secondary amine (PSA);
- removing the absorbent by filtering the mixture through a cellulose filter paper having a particle size of 0.45-0.8 μm, followed by filtering the mixture again through a cellulose filter paper having a particle size of 0.2 μm under vacuum, to produce a purified bee venom solution free of water-soluble foreign impurities;
- and
- lyophilizing the purified bee venom solution at −20° C. or less to obtain said purified bee venom, wherein the purified bee venom is 99% pure.
Dependencies are inferred from the claim text.