Back to results
US 9,229,009 B2Granted
Methods and kits for detecting misfolded proteins
AssigneesYale University, The Regents of the University of California
InventorsCatalin S. Buhimschi, Irina Buhimschi, Charles G. Glabe
Life of this patent
Estimating the term…
Abstract
Methods, kits and compounds are provided that relate to the diagnosis, treatment, and/or prevention of preeclampsia.
Claims11
A method of detecting misfolded protein aggregates in urine obtained from a pregnant woman, comprising:
- (a) combining a urine sample obtained from a pregnant woman with Congo Red under conditions under which Congo Red stains proteins that are components of protein aggregates in the urine sample, thereby producing a urine sample that comprises Congo Red-stained proteins;
- (b) applying the urine sample that comprises Congo Red-stained proteins to a surface that has affinity to proteins, under conditions under which proteins bind to the surface and produce a Congo Red-stained surface;
- and
- (c) detecting staining intensity of the area of Congo Red-stained surface, wherein misfolded protein aggregates are detected if the staining intensity is greater than a negative control sample or comparable to a positive control sample.
1 more element
A method of detecting misfolded protein aggregates in urine obtained from a pregnant woman, comprising:
- (a) combining a urine sample obtained from a pregnant woman with Congo Red under conditions under which Congo Red stains proteins that are components of protein aggregates in the urine sample, thereby producing a urine sample that comprises Congo Red-stained proteins;
- (b) applying the urine sample that comprises Congo Red-stained proteins to a surface that has affinity to proteins under conditions under which proteins bind to the surface and produces a Congo Red-stained surface;
- (c) washing the area of stained surface under conditions that remove Congo Red that has not stained protein;
- and
- (d) detecting staining intensity of the area of stained surface, wherein misfolded protein aggregates are detected if the staining intensity is greater than a negative control sample or comparable to a positive control sample.
2 more elements
A nitrocellulose membrane comprising a spot that comprises
- urine obtained from a pregnant woman,
- misfolded protein aggregates and
- Congo Red.
Dependencies are inferred from the claim text.