US 9,220,810 B2 · Mesenchymal stem cells (MSC) expansion methods and materials

Abstract

The subject invention concerns materials and methods for growing and expanding MSC while maintaining their undifferentiated phenotype, self-renewal ability, and/or multi-lineage potential. In one embodiment, a method of the invention comprises i) seeding freshly isolated MSC on a planar surface or a 3-D scaffold and growing the cells under physiological or low O2 tension for a period of time sufficient to support formation of 3-D ECM network; ii) decellularizing the planar surface or 3-D scaffold; and iii) reseeding the decellularized planar surface or 3-D scaffold with MSCs, whereby the reseeded MSCs can be grown on the scaffold and maintain an undifferentiated phenotype. In one embodiment, the 3-D scaffold comprises or is composed of PET. In one embodiment, the MSC are human MSC (hMSC).

Claims22

Dependent claims

A method for growing and/or expanding mammalian mesenchymal stem cell (MSC), the method comprising:

a) seeding freshly isolated mammalian MSC on a planar surface or a porous three-dimension (3-D) scaffold, wherein said surface or scaffold comprises one or more thermally responsive films or coatings;
b) growing said mammalian MSC under an O2 tension that is lower than 20%;
c) decellularizing said surface or scaffold;
and
d) reseeding said surface or scaffold with freshly isolated mammalian MSC, whereby said reseeded mammalian MSC grow and maintain an undifferentiated phenotype.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

13/323,475Filed 12 Dec 2011 · us provisional application

Priority

10 Dec 2010

Published

US 2012/0178159 A112 Jul 2012

Granted

29 Dec 2015

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

A61L 27/3804
Containing added animal cells: characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
A61L 27/3895
Containing added animal cells: using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
C12N 5/0062
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: general…
C12N 5/0663
Stem cells: bone marrow mesenchymal stem cells

Mesenchymal stem cells (MSC) expansion methods and materials

Life of this patent

Mesenchymal stem cells (MSC) expansion methods and materials

Life of this patent

Abstract

Claims22