US 9,217,154 B2Granted

Actinomycete integrative and conjugative element from Actinoplanes sp. SE50/110 as plasmid for genetic transformation of related Actinobacteria

AssigneeBayer Intellectual Property GmbH

InventorsAndreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier

Life of this patent

Estimating the term…

patentgrep

Loading patent…

Abstract

The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.

Claims4

Dependent claims

A host cell transformed with a heterologous polynucleotide having the sequence of SEQ ID NO:1.

Dependencies are inferred from the claim text.

Facts

Application: 14/363,810Filed 4 Dec 2012
Priority: EP 111926188 Dec 2011
Published: US 2014/0349346 A127 Nov 2014
Granted: 22 Dec 2015
Term ends: Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12P 19/26
Preparation of compounds containing saccharide radicals: preparation of nitrogen-containing carbohydrates
C07K 14/365
From bacteria: from Actinoplanes
C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
C12N 15/74
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…