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US 9,216,414 B2Granted
Microfluidic devices and methods for gene synthesis
AssigneeGen9, Inc.
InventorLarry Li-Yang Chu
Life of this patent
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Abstract
Certain aspects of the present invention provide devices and methods for preparing oligonucleotides and for assembling nucleic acid molecules using microfluidic devices.
Claims20
A microfluidic system comprising:
- a continuous carrier phase fluid comprising droplets that are immiscible with the carrier phase fluid;
- at least one main channel adapted to carry the carrier phase fluid, the channel comprising interior walls;
- and
- a plurality of defined surface-capture spots forming a pattern on the channel interior walls, at least one of the plurality of defined surface-capture spots comprising oligonucleotides and a surface area modification having a hydrophilic surface property different than the interior walls, wherein the hydrophilic surface property allows droplet absorption and/or attachment, the plurality of defined surface-capture spots comprising oligonucleotides being configured to allow assembly of the oligonucleotides into a target polynucleotide sequence.
1 more element
A microfluidic system for assembling a target polynucleotide having a predetermined sequence comprising:
- a continuous carrier phase fluid comprising droplets that are immiscible with the carrier phase fluid;
- a plurality of channels adapted to carry the carrier phase fluid, the channel comprising hydrophobic interior walls;
- a plurality of defined surface-capture spots on the interior walls, the surface-capture spots being chemically modified to confer hydrophilic surface property that allows droplet absorption and/or attachment, each defined surface-capture spot comprising oligonucleotides having a different predetermined subsequence that together form the predetermined sequence of the target polynucleotide;
- and
- a force transducer for controlling droplet displacement from a first defined surface-capture spot to a second defined surface-capture spot to allow assembly of the oligonucleotides into the target polynucleotide.
2 more elements
Dependencies are inferred from the claim text.