Lipoparticles comprising proteins, methods of making, and using the same
AssigneeIntegral Molecular, Inc.
InventorsBenjamin J. Doranz, Sharon Willis
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Abstract
The present invention relates to lipoparticles. The invention also relates to producing lipoparticles. The invention further relates to lipoparticles comprising a viral structural protein. The invention further relates to a lipoparticle comprising a membrane protein, and the lipoparticle can be attached to a sensor surface. The invention further relates to methods of producing and using the lipoparticle to, inter alia, assess protein binding interactions.
Claims20
A method of identifying a binding partner of a multiple membrane spanning protein of interest comprising:
- a) contacting a test binding partner with a lipoparticle, the lipoparticle comprising
- an external lipid bilayer;
- an enveloped retroviral structural protein;
- and
- the multiple membrane spanning protein of interest,
- wherein said enveloped retroviral structural protein is an uncleaved gag protein,
- wherein said gag protein does not comprise a heterologous tag that binds to the multiple membrane spanning protein,
- provided that the only viral proteins in the lipoparticle are structural proteins
- b) detecting the presence of binding of the test binding partner to said membrane protein,
- wherein the presence of binding identifies the test binding partner as a binding partner to said membrane protein of interest.
A method of identifying a binding partner of a multiple membrane spanning protein of interest comprising:
- a) contacting a library of test binding partners with a lipoparticle, the lipoparticle comprising:
- an external lipid bilayer;
- an enveloped retroviral structural protein;
- and
- the multiple membrane spanning protein of interest,
- wherein said enveloped retroviral structural protein is an uncleaved gag protein,
- wherein said gag protein does not comprise a heterologous tag that binds to the multiple membrane spanning protein,
- provided that the only viral proteins in the lipoparticle are structural proteins
- b) detecting the presence of binding of the test binding partner to said membrane protein,
- wherein the presence of binding identifies a member of the library as a binding partner to said membrane protein of interest.
Dependencies are inferred from the claim text.