Nutrient monitoring and feedback control for increased bioproduct production
AssigneeBiogen Idec MA Inc.
InventorsValerie Liu Tsang, Angela Xiaoying Wang, Helena Yusuf-Makagiansar
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Abstract
The present invention pertains to methods of increasing the efficiency of producing a bioproduct. In some embodiments, the method increases the quantity of a bioproduct produced, or decreases bioproduct production time, in a bioreactor cell culture producing the bioproduct, the method comprising, (a) intermittently or continuously analyzing the concentration of one or more nutrients in the bioreactor cell culture; and (b) adding to the bioreactor cell culture additional nutrient media when the concentration of the one or more nutrients is lower than a target value.
Claims16
An automated method of increasing the quantity of a bioproduct produced, or decreasing bioproduct production time, in a bioreactor cell culture process, the method comprising:
- (a) intermittently or continuously analyzing one or more carbohydrates or vitamins in the bioreactor cell culture by means of an automated sampling device to generate data representative of the concentration of said one or more carbohydrates or vitamins;
- (b) processing said data by means of a computer-based processing program to determine an amount of nutrient media to add to the bioreactor;
- and,
- (c) adding said amount of nutrient media determined in (b) to the bioreactor cell culture by means of an automated feed device when the concentration of said one or more carbohydrates or vitamins is below a target value.
An automated method of enhancing the quantity of a bioproduct produced, or decreasing bioproduct production time, in a bioreactor cell culture producing the bioproduct, the method comprising:
- (a) performing a first bioreactor cell culture process by:
- (i) analyzing a bioreactor sample by means of an automated analytical device to generate data representative of the quantity of one or more carbohydrates or vitamins;
- (ii) processing said data by means of computer-based processing program to determine an amount of nutrient media to add to the bioreactor;
- (iii) adding said amount of nutrient media determined in (ii) to the bioreactor cell culture by means of an automated feed device when the quantity of said one or more carbohydrates or vitamins is below a target value;
- and
- (iv) recording the time and amount of each nutrient media addition;
- and
- (b) performing a subsequent bioreactor cell culture process, wherein the time and amount of each nutrient media addition in the subsequent bioreactor cell culture process is determined based on the time and amount of each nutrient media addition in the first bioreactor cell culture process.
Dependencies are inferred from the claim text.