Utilization of pharmacological chaperones to improve manufacturing and purification of biologics
AssigneeAmicus Therapeutics, Inc.
InventorHung V. Do
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Abstract
The present invention provides methods for improving the production of recombinant proteins through the use of pharmacological chaperones for the recombinant proteins. As exemplified by the present invention, the binding of a pharmacological chaperone to a recombinant protein expressed by a cell can stabilize the protein and increase export of the protein out of the cell's endoplasmic reticulum, and increase secretion of the protein by the cell.
Claims7
A method for improving the yield of an active recombinant human wild-type acid β-Glucosidase protein expressed by a host cell, wherein the host cell is in media having a pH greater than 5.0, which method comprises
- contacting the host cell in vitro with isofagomine, wherein contacting the host cell with isofagomine increases activity of the acid β-Glucosidase secreted out of the cell, and
- purifying the secreted acid β-Glucosidase from the media, wherein the isofagomine is contacted to the host cell in an amount effective to increase the yield stability of the active acid β-Glucosidase during purification,
- wherein the isofagomine is present at a concentration of at least 3 μM.
Dependencies are inferred from the claim text.