Fucosylation-deficient cells
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsGang Chen, Darya Burakov, James P. Fandl
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Abstract
An isolated nucleic acid encoding an FX protein having a serine at position 79, a lysine at position 90, a leucine at position 136, an arginine at position 211, a serine at position 289, and a combination thereof is provided. Cells having a gene encoding a modified FX protein are provided, wherein the cells exhibit a reduced ability to fucosylate a glycoprotein at a first temperature, but exhibit the ability to fucosylate the glycoprotein at a second temperature. Methods and compositions for making glycoproteins with reduced fucosylation are provided.
Claims12
A cell capable of fucosylating a glycoprotein, wherein the cell comprises a nucleic acid encoding a modified GDP-4-keto-6-deoxy-mannose-3,5-epimerase-4-reductase (FX) protein consisting of SEQ ID NO:1 except having an amino acid modification serine at position 289 (289S), and wherein no more than about 10% of the glycoprotein is fucosylated when the cell is grown at a temperature of about 37° C. in the absence of an external fucose source.
An isolated nucleic acid encoding a modified GDP-4-keto-6-deoxy-mannose-3,5-epimerase-4-reductase (FX) protein consisting of SEQ ID NO:1 except having an amino acid modification serine at position 289 (289S).
Dependencies are inferred from the claim text.