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US 9,206,449 B2Granted
Process of biologically producing a p-hydroxybenzoic acid
AssigneeSamsung Electronics Co., Ltd.
InventorsWon Jae Choi, Jong Won Byun, Jin Ho Ahn, Young Wan Ha, Joo-Hyun Seo
Life of this patent
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Abstract
A method of biologically producing p-hydroxybenzoic acid, and a method for producing p-hydroxybenzoic acid from lignin through chemical and biological conversion.
Claims12
A method for biologically producing p-hydroxybenzoic acid, comprising:
- contacting a substrate with a biocatalyst, wherein the substrate is a lignin-degradation product that comprises an aromatic carboxylic acid having (a) a p-hydroxy group and (b) a hydroxyl group or C1-C4 alkoxy group attached to at least one other position of the aromatic ring, and the biocatalyst comprises a purified enzyme that removes the hydroxyl group or C1-C4 alkoxy group of (b) to produce p-hydroxybenzoic acid;
- a recombinant microorganism transformed with an expression vector encoding the enzyme;
- or a lysate of the recombinant microorganism, or an extract of the recombinant microorganism lysate, comprising the enzyme
- wherein the enzyme is anthranilate synthase (EC 4.1.3.27), 5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate-lyase (EC 4.2.3.5), chorismate lyase (EC 4.1.3.40), 3-dehydroshikimate hydrolyase (EC 4.2.1.118), isochorismate lyase (EC 4.2.99.21), aminodeoxychorismate lyase (EC 4.1.3.38), 3-dehydroquinate hydro-lyase (EC 4.2.1.10), prephenate hydro-lyase (EC 4.2.1.51), or hydroxyphenylpyruyate synthase (EC 5.4.99.5).
1 more element
A method for biologically producing p-hydroxybenzoic acid, the method comprising:
- degrading lignin into a degraded product comprising an aromatic carboxylic acid having (a) a p-hydroxy group and (b) a hydroxy group or C1-C4 alkoxy group attached to at least one other position of the aromatic ring;
- and
- contacting the degraded product with a biocatalyst;
- wherein the biocatalyst comprises a purified˜enzyme that removes the hydroxyl group or C1-C4 alkoxy group of (b) to produce p-hydroxybenzoic acid;
- a recombinant microorganism transformed with an expression vector encoding the enzyme;
- or a lysate of the recombinant a microorganism, or an extract of the recombinant microorganism lysate, comprising the enzyme;
- wherein the enzyme is anthranilate synthase (EC 4.1.3.27), 5-O-(1-carboxvvinyl)-3-phosphoshikimate phosphate-lyase (EC 4.2.3.5), chorismate lyase (EC 4.1.3.40), 3-dehydroshikimate hydrolyase (EC 4.2.1.118), isochorismate lyase (EC 4.2.99.21), aminodeoxychorismate lyase (EC 4.1.3.38), 3-dehydrominate hydro-lyase (EC 4.2.1.10), prephenate hydro-lyase (EC 4.2.1.51), or hydroxyphenylpyruvate synthase (EC 5.4.99.5).
4 more elements
Dependencies are inferred from the claim text.