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AssigneeSolazyme, Inc.
InventorsScott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
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Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.
A triglyceride oil produced by a microalgae, the oil having an altered fatty acid profile relative to an oil produced by a non-recombinant microalgae, comprising 3.5% or less saturated fatty acids, greater than 50% oleic acid, and optionally comprising greater than 1% palmitoleic acid, wherein the microalgae is a recombinant oleaginous cell of the genus Prototheca, comprising an exogenous gene encoding an active ketoacyl-ACP synthase.
Dependencies are inferred from the claim text.