Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

A detection apparatus, comprising

• (a) a carriage comprising a plurality of microfluorometers,
• wherein the plurality of microfluorometers form a read head,
• wherein the microfluorometers are permanently fixed in the read head such that the microfluorometers are not independently moveable with respect to each other in a direction parallel to the common plane,
• wherein the read head comprises a co-molded assembly of the plurality of microfluorometers,
• wherein each of the microfluorometers comprises an objective having a field diameter of at least 1 mm,
• wherein the plurality of microfluorometers is positioned to simultaneously acquire a plurality of wide-field images in a common plane, and
• wherein each of the wide-field images is from a different area of the common plane;
• (b) a translation stage configured to move the carriage in at least one direction parallel to the common plane;
• and
• (c) a sample stage configured to hold a substrate in the common plane.