Allografts combined with tissue derived stem cells for bone healing
AssigneeAlloSource
InventorYaling Shi
Life of this patent
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Abstract
There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate and the MSCs are allowed to adhere. The substrate is rinsed to remove unwanted cells. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate made according to the described method. Other embodiments are also disclosed.
Claims64
A method of making an allograft product for enhancing bone formation, the method consisting of:
- providing a bone substrate obtained from a human, cadaveric donor;
- providing adipose tissue comprising mesenchymal stem cells and unwanted cells from the human, cadaveric donor;
- digesting and centrifuging the adipose tissue to form a stromal vascular fraction pellet comprising the mesenchymal stem cells and the unwanted cells;
- adding the stromal vascular fraction pellet to the bone substrate to form a seeded bone substrate;
- incubating the seeded bone substrate for a period of time to allow the mesenchymal stem cells to adhere to the bone substrate;
- and
- rinsing the seeded bone substrate to remove the unwanted cells from the bone substrate;
- thereby making the allograft product for enhancing bone formation, wherein the allograft product comprises bone substrate with mesenchymal stem cells adhered thereto.
A method of making an allograft product for enhancing bone formation, the method consisting of:
- providing a bone substrate obtained from a human, cadaveric donor;
- providing an adipose stromal vascular fraction pellet obtained by digesting and centrifuging adipose tissue from the human, cadaveric donor, the adipose stromal vascular fraction pellet comprising mesenchymal stem cells and unwanted cells;
- adding the stromal vascular fraction pellet to the bone substrate to form a seeded bone substrate;
- incubating the seeded bone substrate for a period of time to allow the mesenchymal stem cells to adhere to the bone substrate;
- and
- rinsing the seeded bone substrate to remove the unwanted cells from the bone substrate;
- thereby making the allograft product for enhancing bone formation, wherein the allograft product comprises bone substrate with mesenchymal stem cells adhered thereto.
A method of making an allograft product for enhancing bone formation, the method consisting of:
- providing a bone substrate obtained from a human, cadaveric donor;
- providing adipose tissue comprising mesenchymal stem cells and unwanted cells from the human, cadaveric donor;
- digesting and centrifuging the adipose tissue to form a stromal vascular fraction pellet comprising the mesenchymal stem cells and the unwanted cells;
- adding the stromal vascular fraction pellet to the bone substrate to form a seeded bone substrate;
- incubating the seeded bone substrate for a period of time to allow the mesenchymal stem cells to adhere to the bone substrate;
- rinsing the seeded bone substrate to remove the unwanted cells from the bone substrate;
- and
- placing the seeded bone substrate into a cryopreservation medium;
- thereby making the allograft product for enhancing bone formation, wherein the allograft product comprises bone substrate with mesenchymal stem cells adhered thereto.
A method of making an allograft product for enhancing bone formation, the method consisting of:
- providing a bone substrate obtained from a human, cadaveric donor;
- providing an adipose stromal vascular fraction pellet obtained by digesting and centrifuging adipose tissue from the human, cadaveric donor, the adipose stromal vascular fraction pellet comprising mesenchymal stem cells and unwanted cells;
- adding the stromal vascular fraction pellet to the bone substrate to form a seeded bone substrate;
- incubating the seeded bone substrate for a period of time to allow the mesenchymal stem cells to adhere to the bone substrate;
- rinsing the seeded bone substrate to remove the unwanted cells from the bone substrate;
- and
- placing the seeded bone substrate into a cryopreservation medium;
- thereby making the allograft product for enhancing bone formation, wherein the allograft product comprises bone substrate with mesenchymal stem cells adhered thereto.
Dependencies are inferred from the claim text.